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TOXICOLOGICAL AND BIOCHEMICAL IMPACT OF SOME BIOINSECTICIDES AGAINST THE LARVAE OF THE RED PALM WEEVIL, Rhynchophorus ferrugineus (OLIVIER) UNDER LABORATORY CONDITIONS |  UTTAR PRADESH JOURNAL OF ZOOLOGY

The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae), is a major pest of date palms, inflicting significant economic damage. This pest has been managed using a variety of control approaches. The intensive use of conventional insecticides to control RPW has successfully reduced the quantity of weevils, but they are still hazardous to the ecosystem since they create pollution and harm to other beneficial organisms. The current study sought suitable, effective, and safe alternative control methods. Furthermore, the effect of the studied substances on the enzymatic activity of third instar larvae was measured spectrophotometrically. Under laboratory circumstances, four commercial pesticides were treated to RPW 3rd instar larvae and the LC50 values were estimated. Larvae that survived treatment were collected 24 hours later and prepared for enzymatic activity examination. All experiments were conducted at the Wood and tree scavenger research department, the Plant protection research institute, and the Agricultural research centre. According to the low LC50 value obtained, Dr. Sure® was the most hazardous chemical. Furthermore, the results demonstrated that BIO-MAGIC® was the least harmful due to its high LC50 value when compared to the other chemicals. Furthermore, the data demonstrated substantial effects on detoxifying enzymes in 3rd instar larvae fed with the LC50 of examined chemicals as a protective response to those compounds. These findings demonstrate the effectiveness of non-conventional pesticides in controlling the youngest larval instars.

Please see the link :- http://mbimph.com/index.php/UPJOZ/article/view/2524


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STRESS MARKERS AND BIOCHEMICAL ALTERATIONS DUE TO CAMINUM NANOPARTICLES EXPOSURE IN FISH Cirrhinus mrigala | UTTAR PRADESH JOURNAL OF ZOOLOGY

The present investigation carried out to determine the effect of cadmium Nano Particles (Cd NPs) on stress bio-markers assay in Cirrhinus mrigala (C. mrigala) fish fingerlings. The nanoparticles (NP) in fishes carried through the use of ion transporters or by endocytosis. Aquatic metal exposure may cause the deleterious effects to all aquatic organisms. The previous studies have shown that the NP were found to deposit in the liver of fishes. The C. mrigala fish fingerlings were acclimatized to laboratory conditions for 10days at a water temperature of 27 ± 2°C, before starting the experiment. They were fed ad libitum regular fish feed two times a day in the morning and evening. Bio-assays were carried out for 10 days.

Please read full article – http://mbimph.com/index.php/UPJOZ/

STRESS MARKERS AND BIOCHEMICAL ALTERATIONS DUE TO CAMINUM NANOPARTICLES EXPOSURE IN FISH Cirrhinus mrigala | UTTAR PRADESH JOURNAL OF ZOOLOGY

The present investigation carried out to determine the effect of cadmium Nano Particles (Cd NPs) on stress bio-markers assay in Cirrhinus mrigala (C. mrigala) fish fingerlings. The nanoparticles (NP) in fishes carried through the use of ion transporters or by endocytosis. Aquatic metal exposure may cause the deleterious effects to all aquatic organisms. The previous studies have shown that the NP were found to deposit in the liver of fishes. The C. mrigala fish fingerlings were acclimatized to laboratory conditions for 10days at a water temperature of 27 ± 2°C, before starting the experiment. They were fed ad libitum regular fish feed two times a day in the morning and evening. Bio-assays were carried out for 10 days. It was revealed that the LC50 value of Cd NP as 50 ppm in C. mrigala. A series of five different concentrations such as 20, 40, 60, 80,100 ppm of CdNP suspension with 100 nm in size was mixed intra peritoneally per kg of fish weight. The biochemical enzyme studies (i.e.) alkaline phosphatase, Lactate dehydrogenase (LDH) and Malate dehydrogenase (MDH) were estimated by standard procedures in tissues such as liver, and kidney. However, enzymes LDH and MDH showed the biochemical changes during the stress condition in the fish on nanoparticles exposure. The outcome result data were tabulated and statistically analyzed by Statistical Package for the Social Sciences version 16 (SPSS), one-way ANOVA (Analysis of Variance) used to observe the treatment effect. The present study reveals that the MDH enzyme level in the 2nd and 10th day in liver and kidney were 0.54±0.60; 0.9±0.15 and 0.24± 0.48; 0.41± 0.07 respectively. On 10th day the alkaline phosphatase intensity in the liver and kidney were 10.45± 03 and 10.24± 0.80 respectively.

Please read full article – http://mbimph.com/index.php/UPJOZ/article/view/1477

Keywords – Cadmium nanoparticles, acute toxicity, fish fingerlings, C. mrigala, stress markers, alkaline phosphatase, lactate dehydrogenase and malate dehydrogenase.